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KMID : 0385220010110020021
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Korean Journal of Gerontology
2001 Volume.11 No. 2 p.21 ~ p.27
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Effects of 17β-estradiol on TNFα-or Tamoxifen-induced Apoptosis in Human Breast Cancer Cells
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Jo Un-Bock
Hong ki-Whan
Kim Byeong-Gee
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Abstract
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E2 has been known to induce cell proliferation of the estrogen receptor (ER)-positive MCF-7 human breast cancer cell line. Besides its growth stimulating mechanism through ER, E2 has been documented to exert antioxidant effects and antiapoptotic effects in recent studies. In the present experiments, the antiapoptotic effects of E2 on TNFα or TAM-induced apoptosis were compared in ER-positive MCF-7 cells and ER-negative MDA-MB-231 cells. TNFα and TAM had significant dose- and time- dependent inhibitory effects on the growth of MCF-7 cells. However, the proliferation of MDA-MB-231 cells was not inhibited by either TNFα or TAM treatment. The generation of ROS was also increased in dose- and time- dependent manners by TNFα treatment in MCF-7 cells. However, TAM had no effect on ROS generation in MCF-7 cells. E2 suppressed TNFα-induced ROS generation in ER-positive MCF-7 cells. As documented in other cell systems, the antioxidant effect of E2 might be receptor-independent. In MDA-MB-231 cells, however, neither TNFα nor TAM could induce ROS generation. Antioxidant effects of E2 were also demonstrated by DNA fragmentation, cell morphology change, and ROS generation assessment. E2 also blocked the TAM-induced cell death in MCF-7 cell. These findings demonstrate that E2 can prevent TNFα or TAM-induced apoptosis in ER-positive MCF-7 human breast cancer cells.
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KEYWORD
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TNFα, Tamoxifen, MCF-7 cells, MDA-MB-231 cells
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